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	Provedor de dados BABT (1) Autor Kaur,Hardeep (1) Kumar,Sachin (1) Verma,Neelam (1) Palavra-chave Biosensor (1) Hydrosol- gel immobilization (1) Lead (1) Masking agent (1) Milk (1) Urease (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Ano 2014 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Enzyme-based Colorimetric and Potentiometric Biosensor for Detecting Pb (II) Ions in Milk BABT Kaur,Hardeep; Kumar,Sachin; Verma,Neelam. The aim of the present work was to study a simple colorimetric and potentiometric biosensor based on urease inhibition by Pb (II) ions for its estimation in milk samples. Urease immobilized on nylon membrane by hydrosol gel method was used as the biocomponent to demonstrate the metal effect on the enzyme activity using phenol red as the pH indicator. A lower limit detection of 38.6µm was achieved in the milk and the enzyme membranes were stable for more than two months at 4ºC. In potentiometric approach, response of an ion selective electrode (ISE) to changing ammonium ion concentration as a consequence of urease inhibition by Pb (II) ions was explored to achieve a detection limit of 9.66 µm. Lead specificity was attained by means of masking agents 1,10 -... Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosensor; Urease; Hydrosol- gel immobilization; Lead; Milk; Masking agent. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400613 Registros recuperados: 1 Primeira ... 1 ... Última

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